Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of CCT3 (arrow) using rabbit polyclonal CCT3 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2).
Western blot of CCT3 Antibody in MCF7,CEM,K562,HL-60,HeLa cell line lysates (35 ug/lane). CCT3 (arrow) was detected using the purified antibody.
Flow cytometric of K562 cells using CCT3 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.