Western blot analysis of extracts from U-2 OS cells synchronized in mitosis with Nocodazole #2190 (100 ng/ml, 18 hr) and released into fresh medium for the indicated times, using Phospho-FoxM1 (Ser35) Antibody (upper), FoxM1 (D12D5) XP ® Rabbit mAb #5436 (middle) or α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower).
Western blot analysis of extracts from HeLa cells that are untreated (-), synchronized in S-phase by double thymidine block (2 mM, 18 hr; +), or synchronized in mitosis with double thymidine block followed by release into fresh medium containing Nocodazole #2190 (100 ng/ml, 18 hr; +), using Phospho-FoxM1 (Ser35) Antibody (upper), FoxM1 (D12D5) XP ® Rabbit mAb #5436 (middle) or α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower).
Western blot analysis of extracts from ACHN cells that are untreated (-) or synchronized in mitosis with Nocodazole #2190 (100 ng/ml, 18 hr; +), using Phospho-FoxM1 (Ser35) Antibody (upper), FoxM1 (D12D5) XP ® Rabbit mAb #5436 (middle) or α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower).