Phospho-FRA1 (Ser265) (D22B1) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 5841
• Prices: $109.00, $287.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D22B1
• Applications: WB ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Bovine, Horse
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser265 of human FRA1 protein.
• Description: Rabbit Monoclonal
• Gene: FOSL1

Product images

Western blot analysis of extracts from HeLa cells, serum-starved overnight, and either left untreated or treated with TPA #4174 for 4 hours, using Phospho-FRA1 (Ser265) (D22B1) Rabbit mAb #5841 (upper) and FRA1 (D80B4) Rabbit mAb #5281 (lower). Antibody phospho-specificity is shown by treating lysates with λ phosphatase.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and either 10 μl of Phospho-FRA1 (Ser265) (D22B1) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR SimpleChIP ® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP ® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.