Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 K562 cells and either 10 μl of G9a/EHMT2 (C6H3) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human ZNF19 Intron 3 Primers #98428, human VRK3 intron 11 primers, and SimpleChIP ® Human GAPDH Exon 1 Primers #5516. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.