Western blot analysis of cell extracts from serum-starved T-47D cells, untreated (-) or treated with neuregulin (100 ng/ml, 15 min; +), using Phospho-Gab1 (Tyr659) Antibody (upper) or Gab1 Antibody #3232 (lower).
Immunoprecipitation of phospho-Gab1 (Tyr659) from neuregulin-stimulated (100 ng/ml, 15 min) T-47D cell extracts using Normal Rabbit IgG #2729 (lane 2) or Phospho-Gab1 (Tyr659) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Gab1 (Tyr659) Antibody.