Anti-CENP-Q Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy using protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
Anti-CENP-Q Antibody - Western Blot. Western blot of protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in a HeLa whole cell lysate (lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5ug/mL, followed by washes and incubation with to the secondary antibody. Lane 1: Lysates from HeLa cells transfected with control sh-virus. Lane 2: Lysates from HeLa cells transfected with Cenp-Q sh-virus. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.