GATA-3 (D13C9) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 5852
• Prices: $109.00, $277.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D13C9
• Applications: WB IHC-P ICC/IF FC ChIP
• Species Reactivities: Human, Mouse, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-3 protein.
• Description: Rabbit Monoclonal
• Gene: GATA3

Product images

Western blot analysis of extracts from CEM and SH-SY5Y cells using GATA-3 (D13C9) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GATA-3 (D13C9) XP ® Rabbit mAb (left) or Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (right).

Immunohistochemical analysis of paraffin-embedded cell pellets, MCF-7 (left) or HUVEC (right), using GATA-3 (D13C9) XP ® Rabbit mAb.

Confocal Immunofluorescent analysis of MCF7 (left) and HUVE (right) cells using GATA-3 (D13C9) XP ® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Flow cytometric analysis of THP-1 cells (blue) and MCF7 cells (green) using GATA-3 (D13C9) XP ® Rabbit mAb.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 NK-92 cells and either 5 μl of GATA-3 (D13C9) XP ® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CCL4 promoter primers, SimpleChIP ® Human IFN-γ Promoter Primers #13051, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.