Western blot analysis of extracts from various cell lines using GM130 (D6B1) XP ® Rabbit mAb.
Immunoprecipitation of GM130 protein from ZR-75-1 cell extracts, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or GM130 (D6B1) XP ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using GM130 (D6B1) XP ® Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Brefeldin A #9972 (200 μM, 30 min; right), using GM130 (D6B1) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).