Detection of Human CSPP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CSPP1 antibody used for WB at 1 ug/ml (A and B) and used for IP at 6 ug/mg lysate. CSPP1 was also immunoprecipitated by rabbit anti-CSPP1 antibody BL12011, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).