Detection of Human CSTF2T/TauCSTF64 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CSTF2T/TauCSTF64 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Detection of Human CSTF2T by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified rabbit anti-CSTF2T antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human CSTF2T by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CSTF2T antibody used for IP at 6 ug/mg lysate. CSTF2T was also immunoprecipitated by a prior lot (-2) of this antibody. For blotting immunoprecipitated CSTF2T, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.