Western blot analysis of extracts from NIH/3T3 cells, lambda-phosphatase or PDGF-treated, using Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb (upper) or GSK-3-beta (27C10) Rabbit mAb #9315 (lower).
Western blot analysis of extracts from PDGF-treated wild-type, GSK-3-alpha (-/-) and GSK-3-beta (-/-) mouse embryonic fibroblasts (MEFs) using Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb (upper) and GSK-3-alpha/beta Antibody (lower). (MEF wild-type, GSK-3-alpha (-/-) and GSK-3-beta (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).