Monoclonal Anti-GRAMD4 antibody produced in mouse from mouse

Name	Monoclonal Anti-GRAMD4 antibody produced in mouse from mouse
Supplier	Sigma-Aldrich
Catalog	SAB4200634
Prices	$350.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	GR4-1
Applications	FC WB ICC/IF
Species Reactivities	Monkey, Human
Purity/Format	purified immunoglobulin
Description	Mouse Monoclonal
Gene	GRAMD4
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2 μg/mL Monoclonal Anti-GRAMD4, Clone: GR4-1 (<B>Cat. No. SAB4200634</B>). The antibody was developed using Goat Anti-Mouse IgG, Cy3<SUP>™</SUP>-conjugate.

Immunofluorescence
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2 μg/mL Monoclonal Anti-GRAMD4, Clone: GR4-1 (Cat. No. SAB4200634). The antibody was developed using Goat Anti-Mouse IgG, Cy3^™-conjugate.

<B>Immunoblotting</B><BR/>Whole extract of Jurkat cells was separated on SDS-PAGE and probed with Monoclonal Anti-GRAMD4, Clone: GR4-1 (<B>Cat. No. SAB4200634</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. 0.5 μg/mL antibody<BR/>2. 0.5 μg/mL antibody+ GRAMD4 Immunizing peptide

Immunoblotting
Whole extract of Jurkat cells was separated on SDS-PAGE and probed with Monoclonal Anti-GRAMD4, Clone: GR4-1 (Cat. No. SAB4200634). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. 0.5 μg/mL antibody
2. 0.5 μg/mL antibody+ GRAMD4 Immunizing peptide

<B>Immunoblotting</B><BR/>Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-GRAMD4, Clone: GR4-1 (<B>Cat. No. SAB4200634</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. COS-7 <BR/>2. JURKAT<BR/>3. HeLa <BR/>4. MCF7

Immunoblotting
Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-GRAMD4, Clone: GR4-1 (Cat. No. SAB4200634). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. COS-7
2. JURKAT
3. HeLa
4. MCF7

<B>Flow Cytometry</B><BR/>1×10<SUP>6</SUP> HeLa cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 10 μg/test Monoclonal Anti-GRAMD4, Clone: GR4-1 (<B>Cat. No. SAB4200634</B>) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.

Flow Cytometry
1×10⁶ HeLa cells were fixed and permeabilized with 2% Paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were labeled with 10 μg/test Monoclonal Anti-GRAMD4, Clone: GR4-1 (Cat. No. SAB4200634) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody, Purple histogram: antibody specific staining.