Immunohistochemistry Analysis:
Representative lot data.
Formalin Fixed Paraffin Embedded (FFPE) human prostate cancer (Fig. 1 & 2) and human breast cancer (Fig. 3 & 4) tissues were processed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:50 dilution of Cat. No. ABC452, Anti-FAM60A . Reactivity was detected using an Anti-Rabbit secondary antibody and HRP-DAB. cytoplasmic staining of tumor cells in human prostate cancer and cytoplasmic staining of tumor/epithelial cells in human breast cancer tissues was observed
Western Blotting Analysis:
Representative lot data.
Transfected FAM60A cell lysate was probed with Cat. No. ABC452, Anti-FAM60A (2.0 µg/mL).
Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates FAM60A (~35 kDa)
Western Blotting Analysis:
Representative lot data.
A549 cell lysate was probed with Cat. No. ABC452, Anti-FAM60A (2.0 µg/mL).
Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates FAM60A (~30 kDa)