Immunohistochemistry Analysis:
Representative lot data.
Formalin Fixed Paraffin Embedded (FFPE) human ovarian (Fig. 1 & 2) tissue was processed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:4,000 dilution of Cat. No. ABT188, Anti-Protocadherin 19. Reactivity was detected using an Anti-Rabbit secondary antibody and HRP-DAB. Staining was observed in human ovarian tissue
Western Blotting Analysis:
Representative lot data.
Human fetal brain (female) tissue lysate was probed with Cat. No. ABT188, Anti-Protocadherin 19 (5.0 µg/mL).
Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Protocadherin 19 (~160 kDa).
Note: An uncharacterized band may appear at ~17 kDa in some lysates.