Western blot analysis of Axl expression in FHs 173We (A,E), CCD-1064Sk (B,F), NIH/3T3 (C,G) and 3611-RF (D,H) whole cell lysates. Antibodies tested include Axl (C-20): sc-1096 (AD) and Axl (M-20): sc-1097 (EH).
Axl siRNA (h): sc-29769. Western blot analysis of Axl expression in non-transfected control (A) and Axl siRNA transfected (B) HeLa cells. Blot probed with Axl (C-20): sc-1096. α-actinin (H-2): sc-17829 used as specificity and loading control.
Axl (C-20): sc-1096. Western blot analysis of Axl expression in non-transfected: sc-117752 (A) and human Axl transfected: sc-114191 (B) 293T whole cell lysates.
Axl (C-20): sc-1096. Western blot analysis of Axl expression in non-transfected 293T: sc-117752 (A), mouse Axl transfected 293T: sc-126473 (B) and Caki-1 (C) whole cell lysates.
Axl (C-20): sc-1096. Western blot analysis of Axl expression in non-transfected: sc-117752 (A) and mouse Axl transfected: sc-126474 (B) 293T whole cell lysates.
Axl (C-20): sc-1096. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Axl (C-20): sc-1096. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.