Immunohistochemistry Analysis:
Representative lot data.
Formalin Fixed Paraffin Embedded (FFPE) human testis (Fig. 1) and human hyperplastic prostate (Fig. 2) tissues were processed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:50 dilution of Cat. No. ABC244, Anti-PATE1. Reactivity was detected using an Anti-Rabbit secondary antibody and HRP-DAB. Cytoplasmic staining of Leydig and germ cells in human testis and cytoplasmic staining of epithelial cells in human hyperplastic prostate tissues was observed
Western Blotting Analysis:
Representative lot data.
Rat testis tissue lysate was probed with Cat. No. ABC244, Anti-PATE1 (1:500 dilution).
Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates PATE1 (~14 kDa)