Western Blot Analysis: Representative lot data. HeLa cell lysate was probed with Anti-MAP1LC3B2 (1 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MAP1LC3B2 (~16 kDa).
Western Blot (SNAP i.d. system) Analysis: Representative lot data. HeLa cell lysate was probed with Anti-MAP1LC3B2 (5 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MAP1LC3B2 (~16 kDa).
Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded colorectal carcinoma tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of Cat. No. AB2970, Anti-MAP1LC3B2. Reactivity was detected using the IHC-Select Detection Kit (Cat. No. DAB050). Staining pattern appears as nuclear (some cells) and diffusive in the plasma membrane.