Anti-Neurogenin-3 (rabbit) Antibody

Name	Anti-Neurogenin-3 (rabbit) Antibody
Supplier	Sigma-Aldrich
Catalog	AB10535
Prices	$319.00
Sizes	ab10535
Clonality	Polyclonal
Isotype	IgG
Applications	WB
Species Reactivities	Mouse
Purity/Format	Unpurified
Description	Polyclonal
Gene	Neurog3
Supplier Page	Shop

Product images

<b>Immunofluorescent Analysis:</b> Staining pattern and morphology in e15.5 mouse pancreas. Fixed frozen tissue, no antigen retrieval. Antibody diluted 1:2000; secondary detection method was fluorescence (alexa488 conjugated donkey anti-rabbit).

Immunofluorescent Analysis: Staining pattern and morphology in e15.5 mouse pancreas. Fixed frozen tissue, no antigen retrieval. Antibody diluted 1:2000; secondary detection method was fluorescence (alexa488 conjugated donkey anti-rabbit).

Immunohistochemistry Analysis: <br />Representative lot data.<br />Paraffin-embedded mouse embryo E12.5+ tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of Cat. No. AB10535, Anti-Neurogenin-3 (rabbit polyclonal). Reactivity was detected using the IHC-Select Detection Kit (Cat. No. DAB050). Immunoreactivity is expressed in embryonic brain in synaptic processes. Staining pattern apears to be cytoplasmic.

Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded mouse embryo E12.5+ tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of Cat. No. AB10535, Anti-Neurogenin-3 (rabbit polyclonal). Reactivity was detected using the IHC-Select Detection Kit (Cat. No. DAB050). Immunoreactivity is expressed in embryonic brain in synaptic processes. Staining pattern apears to be cytoplasmic.

Western Blotting Analysis:<br />Representative lot data.<br />mNgn3 transfected (lane 2) and wildtype (lane 1) rabbit reticulocyte lysates were resolved by electrophoresis, transferred to PVDF membranes and probed with a 1:1,000 dilution of Anti-Neurogenin-3 (rabbit polyclonal) antibody. <br />Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system. <br />Arrow indicates Neurogenin-3 (~32 kDa).

Western Blotting Analysis:
Representative lot data.
mNgn3 transfected (lane 2) and wildtype (lane 1) rabbit reticulocyte lysates were resolved by electrophoresis, transferred to PVDF membranes and probed with a 1:1,000 dilution of Anti-Neurogenin-3 (rabbit polyclonal) antibody.
Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system.
Arrow indicates Neurogenin-3 (~32 kDa).