Western blot analysis of extracts from DO11.10 thymocyte hybridoma cells, untreated (-) or treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (0.2 µM, 1 hr; +) and Ionomycin, Calcium Salt #9995 (0.33 µM, 1 hr; +), using Phospho-HDAC4 (Ser632)/HDAC5 (Ser661)/HDAC7 (Ser486) Antibody (upper). Phospho-specificity of the antibody was determined by treating cell extracts with λ phosphatase. Total HDAC proteins were detected using Histone Deacetylase 4 (HDAC4) Antibody #2072, Histone Deacetylase 5 (HDAC5) Antibody #2082 and Histone Deacetylase 7 (HDAC7) Antibody #2882.