Immunohistochemical of paraffin-embedded H. brain section using DHFR Antibody. Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical of paraffin-embedded H. liver section using DHFR Antibody. Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot of lysates from 293T, HeLa, Jurkat, Y79 cell line , mouse spleen and rat kidney tissue lysate (from left to right) with DHFR Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.