Immunohistochemical staining of human tissue using anti-DLK1 (human), mAb (PF13-3) at 1:500 dilution. A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human umbilical cord (200x, brown color). B. Isotype control with IgG1 (negative control).
Western blot analysis of human Pref-1using anti-DLK1 (human), mAb (PF13-3) at 1: 2,000 dilution. A. . 1. Human Pref-1 Fc protein. 2. Transfected human Pref-1 full length cell lysate (HEK 293). 3. Mock Transfected HEK293 cell lysate. B. . 1. Human Pref-1 Fc protein. 2. Mouse Pref-1 Fc protein. 3. Human DNER Fc protein. 4. Human DLL1 Fc protein. 5. Human DLL3 Fc protein. 6. Human DLL4 Fc protein. 7. Human Jagged1 Fc protein. 8. Human FGF23 Fc protein.
FACS: DLK1-transfected HEK 293(Full length) cells were stained significantly using anti-DLK1 (human), mAb (PF13-3) .