Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb
| Name | Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 3377 |
| Prices | $119.00, $318.00 |
| Sizes | 20 µl (2 western blots), 100 µl (50 tests) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D2C8 |
| Applications | WB ICC/IF FC |
| Species Reactivities | Human, Mouse, Rat, Monkey, Zebrafish |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3. |
| Description | Rabbit Monoclonal |
| Gene | HIST1H3A |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from HeLa cells, either untreated or treated with nocodazole (100 ng/ml for 18 hours), using Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb #3377 (upper) or Histone H3 Antibody #9715 (lower). Phospho-specificity of the antibody is shown by further treatment of the lysate with λ phosphatase.
Confocal immunofluorescent analysis of HeLa cells using Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb versus propidium iodide (DNA content). The boxed population indicates Phospho-Histone H3 (Ser10) positive cells.