Western blot analysis of lysates from HeLa, C6 and NIH/3T3 cells treated for 24 hours with or without nocodazole and also with or without λ phosphatase, using Phospho-Histone H3 (Thr11) Antibody #9764 (upper) or Histone H3 Antibody #9715 (lower).
Confocal immunofluorescent analysis showing positive signal in mitotic HeLa cells, using Phospho-Histone H3 (Thr11) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Flow cytometric analysis of untreated Jurkat cells, using Phospho-Histone H3 (Thr11) Antibody versus propidium iodide (DNA content). The boxed population indicates Phospho-Histone H3 (Thr11)-positive cells.