Acetyl-Histone H3 (Lys18) Antibody

• Supplier: Cell Signaling Technology
• Catalog: 9675
• Prices: $109.00, $287.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IHC-P ChIP
• Species Reactivities: Human, Mouse, Rat
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys18 of human histone H3
• Description: Rabbit Polyclonal
• Gene: HIST1H3A

Product images

Western blot analysis of extracts from NIH/3T3 cells, untreated or TSA-treated, using Acetyl-Histone H3 (Lys18) Antibody.

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder showing nuclear localization using Acetyl-Histone H3 (Lys18) Antibody.

Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or TSA-treated (right), using Acetyl-Histone H3 (Lys18) Antibody. (no counterstain)

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 20 μl of Acetyl-Histone H3 (Lys18) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human GAPDH Exon 1 Primers #5516, SimpleChIP ® Human RPL30 Exon 3 Primers #7014, SimpleChIP ® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.