MEK-1 (C-18): sc-219. Western blot analysis of MEK-1 expression in KNRK (A) and CTLL-2 (B) whole cell lysates.
MEK-1 (C-18): sc-219. Cytoplasmic immunofluorescence staining of methanol-fixed HeLa cells.
ChIP analysis of cofactor occupancy dynamics on the KAI1 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include MEK-1 (C-18): sc-219, MEK kinase-1 (C-22): sc-252, HDAC1 (C-19): sc-6298, HDAC1 (H-11): sc-8410 and HDAC1 (H-11): sc-7872. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Western blot analysis of MEK-1 phosphorylation in untreated (A,C) and treated (B,D) A-431 whole cell lysates. Antibodies tested include p-MEK-1 (B-4): sc-271914 (A,B) and MEK-1 (C-18): sc-219 (C,D).
Western blot analysis of MEK-1 phosphorylation in untreated (A,C) and treated (B,D) A-431 whole cell lysates. Antibodies tested include p-MEK-1 (Ser 298)-R: sc-30635-R (A,B) and MEK-1 (C-18): sc-219 (C,D).
Western blot analysis of MEK-1 phosphorylation in untreated (A,D), EGF treated (B,E) and EGF and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-MEK-1 (B-4): sc-271914 (A,B,C) and MEK-1 (C-18): sc-219 (D,E,F).
MEK-1 (C-18)-G: sc-219-G. Immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic staining of glandular cells.