MEK-1 (H-8)

• Supplier: Santa Cruz Biotechnology
• Catalog: sc-6250
• Prices: $279.00
• Sizes: 200 µg/ml
• Host: Mouse
• Clonality: Monoclonal
• Isotype: mouse IgG 2b
• Applications: WB IP ICC/IF IHC-P ELISA
• Species Reactivities: Mouse, Rat, Human, Bovine
• Antigen: FL (h)
• Description: Mouse Monoclonal
• Gene: MAP2K1

Product images

MEK-1 (H-8): sc-6250. Western blot analysis of MEK-1 expression in AT-3 (A), KNRK (B) and HeLa (C) whole cell lysates.

Western blot analysis of inactive mouse (A,C,E) and active His-tagged human (B,D,F) recombinant MEK-1. Antibodies tested include MEK-1 (H-8): sc-6250 (A,B), p-MEK-1/2 (Ser 218/Ser 222): sc-7995 (C,D), and p-MEK-1/2 (Ser 218/Ser 222)-R: sc-7995-R (E,F).

MEK-1 (H-8): sc-6250. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal rat brain showing intense cytoplasmic staining.

MEK-1 (H-8): sc-6250. Immunofluorescence staining of methanol-fixed AT-3 cells showing cytoplasmic localization.

Western blot analysis of MEK-1 phosphorylation in untreated (A,D), NGF treated (B,E) and NGF and lambda protein phosphatase treated (C,F) PC-12 whole cell lysates. Antibodies tested include p-MEK-1 (Thr 291): sc-101733 (A,B,C) and MEK-1 (H-8): sc-6250 (D,E,F).

Western blot analysis of MEK-1 phosphorylation in untreated (A,D), NGF treated (B,E) and NGF and lambda protein phosphatase treated (C,F) PC-12 whole cell lysates. Antibodies tested include p-MEK-1 (Thr 386): sc-135701 (A,B,C) and MEK-1 (H-8): sc-6250 (D,E,F).

Western blot analysis of MEK-1/2 phosphorylation in untreated (A,D), PDGF treated (B,E) and PDGF and lambda protein phosphatase treated (C,F) NIH/3T3 whole cell lysates. Antibodies tested include p-MEK-1/2 (7E10): sc-81503 (A,B,C) and MEK-1 (H-8): sc-6250 (D,E,F).

Western blot analysis of MEK-1 phosphorylation in untreated (A,D), serum-starved, PMA-treated (B,E), serum-starved, PMA and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-MEK-1/2 (7E10): sc-81503 (A,B,C) and MEK-1 (H-8): sc-6250 (D,E,F).

Western blot analysis of MEK-1 phosphorylation in untreated (A,D), serum-starved, PMA-treated (B,E), serum-starved, PMA and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-MEK-1 (Thr 291): sc-101733 (A,B,C) and MEK-1 (H-8): sc-6250 (D,E,F).

Western blot analysis of MEK-1 phosphorylation in untreated (A,D), serum-starved, PMA-treated (B,E), serum-starved, PMA and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-MEK-1 (47.Ser 222): sc-136542 (A,B,C) and MEK-1 (H-8): sc-6250 (D,E,F).

goat anti-mouse IgG-HRP: sc-2031. Western blot analysis of MEK-1 expression in HeLa whole cell lysate. Antibody tested: MEK-1 (H-8): sc-6250.

goat anti-mouse IgG-HRP: sc-2032. Western blot analysis of MEK-1 expression in HeLa whole cell lysate. Antibody tested: MEK-1 (H-8): sc-6250.

donkey anti-mouse IgG-HRP: sc-2306. Western blot analysis of MEK-1 expression in HeLa whole cell lysate. Antibody tested: MEK-1 (H-8): sc-6250.

donkey anti-mouse IgG-HRP: sc-2318. Western blot analysis of MEK-1 expression in HeLa whole cell lysate. Antibody tested: MEK-1 (H-8): sc-6250.

bovine anti-mouse IgG-HRP: sc-2380. Western blot analysis of MEK-1 expression in HeLa whole cell lysate. Antibody tested: MEK-1 (H-8): sc-6250.

bovine anti-mouse IgG-HRP: sc-2386. Western blot analysis of MEK-1 expression in HeLa whole cell lysate. Antibody tested: MEK-1 (H-8): sc-6250.

Western blot analysis of MEK-1/2 phosphorylation in untreated (A,D), serum starved and PMA treated (B,E) and serum starved, PMA treated and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-MEK-1/2 (Ser 218/Ser 222)-R: sc--7995-R (A,B,C) and MEK-1 (H-8): sc-6250 (D,E,F).