p-FRAP (Ser 2448): sc-101738. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.
Western blot analysis of FRAP phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) HeLa whole cell lysates. Antibodies tested include p-FRAP (Ser 2448): sc-101738 (A,B) and FRAP (30): sc-136269 (C,D).
Western blot analysis of FRAP phosphorylation in untreated (A,D), Calyculin A treated (B,E) and Calyculin A and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-FRAP (Ser 2448): sc-101738 (A,B,C) and FRAP (30): sc-136269 (D,E,F).