Di-Methyl-Histone H3 (Lys9) Antibody

• Supplier: Cell Signaling Technology
• Catalog: 9753
• Prices: $287.00, $678.00
• Sizes: 100 µl (10 western blots), 300 µl (30 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IP ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Drosophila
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which lysine 9 is di-methylated
• Description: Rabbit Polyclonal
• Gene: HIST1H3A

Product images

Western blot analysis of whole cell lysates from HeLa, NIH/3T3, H-4-II-E and COS cells, using Di-Methyl-Histone H3 (Lys9) Antibody.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 20 μl of Di-Methyl-Histone H3 (Lys9) Antibody or 2 μl Normal Rabbit IgG #2729, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human AFM Intron 1 Primers #5098, SimpleChIP ® Human α Satellite Repeat Primers #4486, SimpleChIP ® Human RPL30 Exon 3 Primers #7014, and SimpleChIP ® Human GAPDH Exon 1 Primers #5516. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.