Tri-Methyl-Histone H3 (Lys4) Antibody

• Supplier: Cell Signaling Technology
• Catalog: 9727
• Prices: $287.00, $678.00
• Sizes: 100 µl (10 western blots), 300 µl (30 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IP IHC-P ICC/IF ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Xenopus, Zebrafish
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which lysine 4 is tri-methylated
• Description: Rabbit Polyclonal
• Gene: HIST1H3A

Product images

Western blot analysis of lysates from HeLa, NIH/3T3, C6 and COS cells, using Tri-Methyl-Histone H3 (Lys4) antibody.

Immunohistochemical analysis of paraffin-embedded human colon using Tri-Methyl-Histone H3 (K4) Rabbit Antibody in the presence of non-methyl peptide (left) or K4 tri-methyl peptide (right).

Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Tri-Methyl-Histone H3 (Lys4) Antibody (green, left) compared to an isotype control (right). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 5 μl of Tri-Methyl-Histone H3 (Lys4) Antibody or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by Real-Time PCR, using SimpleChIP ® Human GAPDH Exon 1 Primers #5516, SimpleChIP ® Human RPL30 Exon 3 Primers #7014, SimpleChIP ® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.