Anti-EN2 Antibody (aa243-271)
| Name | Anti-EN2 Antibody (aa243-271) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C100662 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P WB |
| Species Reactivities | Human, Monkey, Mouse, Rat, Guinea Pig, Horse, Pig |
| Purity/Format | Protein A purified |
| Blocking Peptide | EN1 / Engrailed Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | EN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Fluorescent confocal image of HeLa cell stained with EN2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). EN2 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.
Western blot of EN2 antibody in 293 cell line lysates and mouse stomach and lung tissue lysates (35 ug/lane). EN2 (arrow) was detected using the purified antibody.