Acetyl-Histone H4 (Lys5) Antibody

• Supplier: Cell Signaling Technology
• Catalog: 9672
• Prices: $109.00, $287.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IP IHC-P ICC/IF ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Chicken, Drosophila, Xenopus, Zebrafish, Bovine, Pig, Horse, C. elegans
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H4 in which Lys5 is acetylated
• Description: Rabbit Polyclonal
• Gene: HIST1H4A

Product images

Western blot analysis of extracts from various cell lines using Acetyl-Histone H4 (Lys5) Antibody.

Immunohistochemical analysis of paraffin-embedded human breast using Acetyl-Histone H4 (K5) Rabbit Antibody in the presence of non-acetyl peptide (left) or K5 acetyl-peptide (right).

Immunofluorescent analysis of HeLa cells, untreated (left) or treated with Trichostatin A (TSA) #9950 (right), using Acetyl-Histone H4 (Lys5) Antibody (green) and Golgin-97 Antibody (red). Actin filaments were labeled with a dye conjugated phalloidin (pseudocolored blue).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 20 μl of Acetyl-Histone H4 (Lys5) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human GAPDH Exon 1 Primers #5516, SimpleChIP ® Human RPL30 Exon 3 Primers #7014, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.