
Immunohistochemistry Analysis:
Representative lot data.
Formalin Fixed Paraffin Embedded (FFPE) human kidney (Fig. 1) and human liver (Fig. 2) tissues were processed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:500 dilution of Cat. No. MABN457, Anti-Mitochondrial dicarboxylate carrier, clone 1F5.1. Reactivity was detected using an Anti-Mouse secondary antibody and HRP-DAB. Punctate cytoplasmic staining was observed in tubule epithelial cells in human kidney and hepatocytes in human liver tissues

Western Blotting Analysis:
Representative lot data.
Mouse liver tissue lysate was probed with Cat. No. MABN457, Anti-Mitochondrial dicarboxylate carrier, clone 1F5.1 (1.0 µg/mL).
Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Mitochondrial dicarboxylate carrier (~32 kDa)