Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb
| Name | Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 5737 |
| Prices | $287.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D84D2 |
| Applications | WB ChIP |
| Species Reactivities | Human, Mouse, Rat, Monkey, Xenopus, Bovine, Pig |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H4 in which Lys20 is tri-methylated. |
| Description | Rabbit Monoclonal |
| Gene | HIST1H4A |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from HeLa and NIH/3T3 cells using Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 10 μl of Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb, or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human α Satellite Repeat Primers #4486, SimpleChIP ® Human AFM Intron 1 Primers #5098, and SimpleChIP ® Human GAPDH Exon 1 Primers #5516. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.