Formalin-fixed and paraffin-embedded human placenta tissue reacted with EphA3 antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of anti-EphA3 antibody in CHO cell lysate. EphA3 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot of EphA3(arrow) using rabbit polyclonal EphA3 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA3 gene (Lane 2) (Origene Technologies).