Anti-ER Alpha / Estrogen Receptor Antibody (aa452-480)
| Name | Anti-ER Alpha / Estrogen Receptor Antibody (aa452-480) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C157726 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P ICC/IF WB |
| Species Reactivities | Human, Mouse |
| Purity/Format | Protein A purified |
| Blocking Peptide | EPS8L1 Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | ESR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Fluorescent confocal image of MCF-7 cell stained with ESR1 isoform1 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ESR1 isoform1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ESR1 isoform1 immunoreactivity is localized to cytoplasm significantly.
Western blot of ESR1 isoform1 Antibody antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in K562 cell line lysate. ESR1 isoform1 Antibody (arrow) was detected using the purified antibody.