Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ERP29 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of ERP29 (arrow) using rabbit polyclonal ERP29 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2).
Western blot of ERP29 Antibody in A2058,A375,MCF7,NCI-H460,Y79 cell line lysates (35 ug/lane). ERP29 (arrow) was detected using the purified antibody.
ERP29 Antibody flow cytometry of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.