Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with ESRRA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of ESRRA (arrow) using rabbit polyclonal ESRRA Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ESRRA gene.
Western blot of ESRRA Antibody in A375 cell line lysates (35 ug/lane). ESRRA (arrow) was detected using the purified antibody.
Flow cytometric of 293 cells using ESRRA Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.