Confocal immunofluorescent analysis of NIH/3T3 cells, untreated (left) or etoposide-treated (right), double-labeled with Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue) and beta-Tubulin Antibody #2146 (red).
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with Etoposide (25uM overnight; green) using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 647 Conjugate) (solid lines) or concentration-matched
Rabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor ® 647 Conjugate) #2985 (dashed lines).