Western blot analysis of lysates from HeLa, NIH/3T3, C6 and COS cells, using HP1alpha antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HP1alpha Antibody in the presence of control peptide (left) or HP1 alpha blocking peptide #1004 (right).
Confocal immunofluorescent image of HeLa cells labeled with HP1α antibody (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red).
Flow cytometric analysis of untreated HeLa cells, using HP1alpha antibody (blue) compared to a nonspecific negative control antibody (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 mES cells and either 20 µl of HP1α Antibody #2616 or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Mouse MEST Intron 1 Primers #12870, SimpleChIP ® Mouse MEST Promoter Primers #12928, mouse Intracisternal A-Particle (IAP) LTR promoter primers, and mouse primers specific to a non-genic, non-repetitive region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.