Phospho-HSP27 (Ser82) Antibody

• Supplier: Cell Signaling Technology
• Catalog: 2401
• Prices: $287.00, $678.00
• Sizes: 100 µl (10 western blots), 300 µl (30 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IHC-P IHC-F ICC/IF
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic phospho- peptide corresponding to residues surrounding Ser82 of human HSP27
• Description: Rabbit Polyclonal
• Gene: HSPB1

Product images

Western blot analysis of extracts from HeLa cells, using Phospho-HSP27 (Ser82) Antibody (upper) or control HSP27 antibody (lower). HeLa cells were incubated at 42ºC for 0-2 hours as indicated.

Western blot analysis of extracts of various cell lines, using Phospho-HSP27 (Ser82) Antibody #2401 (upper) or control HSP27 Antibody #2402 (lower).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (A,C) or lambda phosphatase-treated (B,D), using Phospho-HSP27 (Ser82) Antibody (A,B) or HSP27 (G31) Monoclonal Antibody #2402 (C,D).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-HSP-27 (Ser82) Antibody in the presence of control peptide (left) or antigen specific peptide (right).

Immunohistochemical analysis of frozen H1650 xenograft, showing cytoplasmic localization using Phospho-HSP27 (Ser82) Rabbit mAb.

Confocal immunofluorescent analysis of HeLa cells, anisomycin-treated (left) or untreated (right), using Phospho-HSP27 (Ser82) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).