HSP27 (G31) Mouse mAb

• Supplier: Cell Signaling Technology
• Catalog: 2402
• Prices: $99.00, $246.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: G31
• Applications: WB IHC-P ICC/IF
• Species Reactivities: Human, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with full length HSP27 protein.
• Description: Mouse Monoclonal
• Gene: HSPB1

Product images

Western blot analysis of extracts from HeLa and COS-7 cells using HSP27 (G31) Mouse mAb.

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® HSP27 siRNA I (+) using HSP27 (G31) Mouse mAb and p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107. The HSP27 (G31) Mouse mAb confirms silencing of HSP27 expression, while the p44/42 MAPK (Erk1/2) (3A7) Mouse mAb is used to control for loading and specificity of HSP27 siRNA.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using HSP27 (G31) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left panels) or lambda phosphatase-treated (right panels), using Phospho-HSP27 (Ser82) Antibody #2401 (upper panels) or HSP27 (G31) Mouse mAb (lower panels).

Confocal immunofluorescent analysis of A549 cells using HSP27 (G31) Mouse mAb (green). Red = Propidium iodide (fluorescent DNA dye).