Detection of Human FCHSD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FCHSD1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FCHSD1 was also immunoprecipitated by rabbit anti-FCHSD1 antibody BL12148, which recognizes an upstream epitope. For blotting immunoprecipitated FCHSD1, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).