Formalin-fixed and paraffin-embedded human lymph tissue reacted with FOXP3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of FOXP3 Antibody in 293(lane 1), Jurkat cell line(lane 2) and mouse liver tissue(lane 3) lysates (35 ug/lane). FOXP3 (arrow) was detected using the purified antibody.
FOXP3 Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.