Formalin-fixed and paraffin-embedded human prostate carcinoma with GAPDH Antibody (C-term R248), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent of GAPDH Antibody (C-term R248) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescent of GAPDH Antibody (C-term R248) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot of lysates from HeLa,HUVEC cell line (from left to right),using GAPDH Antibody (C-term R248). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
Western blot of GAPDH Antibody (C-term R248) in A2058, A375, CEM cell line lysates (35 ug/lane). GAPDH (arrow) was detected using the purified antibody.
Flow cytometric of HepG2 cells using GAPDH Antibody (C-term R248)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.