IHC of paraffin-embedded Carcinoma of prostate tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
Immunoprecipitation(IP) of GBE1 by using monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-GBE1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.