Western blot analysis of EL4 cells, untreated (-) or treated with TPA #4174 (50 ng/ml), Ionomycin, Calcium Salt #9995 (500 ng/ml), and Brefeldin A #9972 (500 ng/ml; overnight; +), using IL-17A (D1X7L) Rabbit mAb (Mouse Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Flow cytometric analysis of EL4 cells, untreated (left) or treated with TPA #4174 (50 ng/ml), Ionomycin, Calcium Salt #9995 (500 ng/ml), and Brefeldin A #9972 (500 ng/ml, overnight; right), using IL-17A (D1X7L) Rabbit mAb (Mouse Specific). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.