Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of stimulation; +), using IL-6 (D5W4V) XP ® Rabbit mAb (Mouse Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of recombinant Mouse His6 Interleukin-6 (m His6 IL-6) #8857 using IL-6 (D5W4V) XP ® Rabbit mAb (Mouse Specific).
Immunoprecipitation of IL-6 from Raw 264.7 cell extracts treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of stimulation) using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or IL-6 (D5W4V) XP ® Rabbit mAb (Mouse Specific) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IL-6 (D5W4V) XP ® Rabbit mAb (Mouse Specific).
Confocal immunofluorescent analysis of Raw 264.7 cells, untreated (left) or treated with LPS (100 ng/ml, 6 hr; right), using IL-6 (D5W4V) XP ® Rabbit mAb (Mouse Specific) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with LPS (100 ng/ml, 6 hr) (green) using IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific). All cells were incubated with Brefeldin A #9972 (300 ng/ml, last 3 hr of treatment). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.