Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of lysates from HeLa, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
(LEFT)Western blot of anti-GJA1 Antibody in CEM cell line lysates (35 ug/lane). GJA1(arrow) was detected using the purified antibody.(RIGHT)Western blot of hGJA1-N121.Connexin in mouse brain tissue lysates (35 ug/lane). GJA1 (arrow) was detected using the purified antibody.