Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 hr; +), using IDO Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human IDO (hIDO; +), using IDO Antibody.
Immunoprecipitation of IDO from HeLa cells, treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 hr), using Normal Rabbit IgG #2729 (lane 2) or IDO Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IDO Antibody.