Detection of Human GKAP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T(15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GKAP1 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GKAP1 was also immunoprecipitated by rabbit anti-GKAP1 antibody BL12181, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).